欧美日日骚,777日韩无码高清影院,嗯啊国产在线观看AAA,超爽内射久久

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2922EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

簡要描述:EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2922
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問  量:2212

產(chǎn)品分類

Product Category

詳細(xì)介紹

EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

ATCC® Number:CRL-2922™    Price:
Designations:EA.hy926

Depositors:CS Edgell

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞


Source:Tissue: somatic cell hybrid

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may gbe required for the transfer of this ATCC material. Anyone purchasin ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
EA.hy926細(xì)胞,人臍靜脈細(xì)胞融合細(xì)胞

Restrictions:For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression:Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR):Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17



Comments:The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C


Subculturing:Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

  6. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

  7. Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.
    Interval: Twice a week
    Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended
    Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete Growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402



References:92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
美女少妇黄色AV| 向日葵视频88亚洲| 欧美成人777777| 三上悠亚亚洲| 中文人妻无码一区二区三区在线| 麻豆精品一区二区三区| 不卡无码av一区二区三区| 人善交video另类HD和按摩| av片亚州| 少妇人妻偷人精品视蜜桃| 欧美国产亚洲欧美| 亚洲国产成人久久精品APP| 美女张开腿黄网站免费久久| 婷婷三级五月开心玫瑰| 好看不卡在线无码AV| 亚洲中文字幕无码久久2020| 亚洲黑人H一区二区| 国产FREEXXXX性播放| 日韩精品福利| 日韩r级网址| 天天做天天摸天天爽天天爱| 亚洲精品无码久久久久看| 中文字母黄色视频片| 噜噜影院高潮| 精品免费视频播放国产| 国产福利啊啊啊啊啊| 久久国产古代三级毛片视频| 1024无码精品| 人妻中文字字幕| 精品国产综合久久精品| 久久久无码精品人妻一区二区| 日韩人妻系列视频| 欧美在线牲交| 久久久久无码高潮| 97国产精品久久久| 无码国模国产在线| 国产精品成aⅴ人片在线观看| a喷亚洲欧美淫爱| 日韩视频免费观看| 国产精品久一二区| 久久精品人成免费|