欧美日日骚,777日韩无码高清影院,嗯啊国产在线观看AAA,超爽内射久久

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CCL-186IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

簡(jiǎn)要描述:IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-186
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-27
  • 訪  問(wèn)  量:2507

產(chǎn)品分類

Product Category

詳細(xì)介紹

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

ATCC® Number:  CCL-186™

Designations:  IMR-90

Depositors:   WW Nichols

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes:  G6PD, B

Age:  16 weeks gestation

Gender:  female

Ethnicity:  Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
精彩刺激对白| 丁香五月综合| 中园老太性饥渴系列| 国产精品免费视频亚洲| 久久av每日更新免费观看| 在线欧美一区| 熟女偷情91视频| 无码高潮呻吟久久久久久| 色妞色综合久久夜夜天天| free性玩弄熟女hd老熟妇| 久久久精亲亚洲天堂网| 日韩欧美另类一区二区导航| 天天曰天天干天天射| 九九九男人天堂| 无码成人动漫一区二区三区| 国产又猛又爽又黄又粗视频| 色先锋影音| 亚洲高清色网站| 国产成人香蕉久久久久| 欧洲精品一区二区亚洲| 日韩av永久| 青青久视频免费观看| 狠狠躁一二三综合网| 丝袜亚洲欧美日韩综合| HEYZO国产亚洲高清| 亚洲无码中文字幕加勒比| 又黄又爽男女视频| 国语国产自产精品| 欧美三级日日日日日日| 欧美日韩亚洲小电影| 日韩V亚洲Ⅴ欧美V精品综合| 丁香婷婷久久久综合精品国产| 国产午夜18久久久| wwwyyy无码| 亚洲妇女水蜜桃av网网站| 欧美大陆国产日韩| www.亚洲涩妇| 熟妇人妻系列AV无码一区二区| 中文亚洲精品无码| 国产一区精品| 色久悠悠2024|